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human embryonic lung fibroblast hfl 1  (ATCC)


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    ATCC human embryonic lung fibroblast hfl 1
    Human Embryonic Lung Fibroblast Hfl 1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 643 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+embryonic+lung+fibroblast+hfl+1/HFL1/pm39921782-101-17-14
    Average 96 stars, based on 643 article reviews
    human embryonic lung fibroblast hfl 1 - by Bioz Stars, 2026-10
    96/100 stars

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    Purification:

    Article Title: A NIR-I light-responsive superoxide radical generator with cancer cell membrane targeting ability for enhanced imaging-guided photodynamic therapy
    Article Snippet: General information 1.1 Materials All general chemicals for organic synthesis and fluorescence detection including 2, 7-dichloro fluorescein diacetate (H2DCF-DA), 9,10-anthracenediylbis (methylene) dimalonic acid (ABDA), dihydrorhodamine 123 (DHR123), superoxide dismutase (SOD), Vitamin C (Vc), Singlet Oxygen Sensor Green (SOSG), TraKine TM Pro Live-cell Tubulin Staining kit (Green Fluorescence dye), Dihydroethidium (DHE), Hydroxyphenyl fluorescein (HPF), 5,5-Dimethyl-1-pyrroline N-oxide (DMPO), dulbecco's modified eagle medium (DMEM), Deoxyribonucleic acid (DNA) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2-(4- Amidinophenyl)-6-indolecar- bamidine dihydrochloride, (DAPI), Mito-Tracker Deep Red, Cell MaskTM Green Plasma Membrane Stain, Calcein - AM/PI Double Stain Kit and apoptosis/necrosis kit (Annexin V-FITC/PI) were obtained from commercial sources (Aladdin, Macklin, Sigma-Aldrich, Bioquest and Thermo). .. Human cervical cancer cell (HeLa cells) and human hepatocellular carcinoma cell (Hep G2) were purchased from BeNa culture collection; human basal cell carcinoma (A431) were obtained from JeDbio corporation; human emborynic kidney (HEK 293T), mouse embryonic fibroblast (3T3), human embryonic lung fibroblast (HFL-1) and Human embryonic lung fibroblast (Helf) were purchased from the American type culture collection; and BALA/c nude mice were given by Shanghai sipul-bikai laboratory animal Co., Ltd. All the other solvents and reagents in this study are of analytical grade and used without further purification. ..

    Transfection:

    Article Title: Innovating non-small cell lung cancer treatment with novel TM-GL/NPs nanoparticles for Glycitin delivery.
    Article Snippet: .. Cell transfection and identification Sourced from American Type Culture Collection (ATCC, USA, https:// www. atcc. org) were human embryonic lung fibroblast HFL-1 (CCL-153), human monocytic macrophage THP-1 (TIB-202), human normal lung epithelial cells BEAS-2B (CRL-3588), human NSCLC cells A549 (CRM-CCL-185) and H1299 (CRL-5803). .. The cultivation of cells involved the use of DMEM medium (D0822, Sigma, USA), 10% fetal bovine serum (FBS) (F8687, Sigma, USA), and 1% antibiotic-mycotic solution (HY-K1058, MCE, USA), within a CO2 incubator set at 37°C.



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